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Home >> Antibodies >> CD1a / HTA1 (Mature Langerhans Cells Marker)

CD1a / HTA1 (Mature Langerhans Cells Marker)

Recombinant Rabbit Monoclonal Antibody [Clone C1A/1506R]

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Catalog No Format Size Price (USD)
909-RBM6-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
909-RBM6-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
909-RBM6-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
909-RBM6-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
909-RBM6-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 909
Human SwissProt P06126
Human Unigene 1309
Human Gene Symbol CD1A
Human Chromosome Location 1q23.1
Synonyms Cortical thymocyte antigen CD1A, Epidermal dendritic cell marker CD1a antibody, FCB6, HTA1, T cell surface antigen T6 / Leu 6, T-Cell Surface Glycoprotein CD1A
Immunogen Recombinant human full-length CD1a protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 49kDa
Cellular Localization Cell surface
Species Reactivity Human. Others not known.
    

Specificity & Comments

At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.

Supplied As

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. McNally, AK. et al. 2011. Exp. Mol. Pathol. 91: 673-681.
  2. Matsuda, A. et al. 2009. Invest. Ophthalmol. Vis. Sci. 50: 2871-2877.
  3. Gulubova, M. et al. 2008. Clin. Exp. Metastasis. 25: 777-785.
  4. Cassaday, RD. et al. 2007. Clin. Cancer Res. 13: 540-549.
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