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Home >> Antibodies >> CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker)

CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker)

Recombinant Rabbit Monoclonal Antibody [Clone Ki-1/1747R]

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Catalog No Format Size Price (USD)
943-RBM7-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
943-RBM7-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
943-RBM7-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
943-RBM7-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
943-RBM7-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 943
Human SwissProt P28908
Human Unigene 1314
Human Gene Symbol TNFRSF8
Human Chromosome Location 1p36.22
Synonyms CD30L receptor, Cytokine receptor CD30, Ki-1 antigen, Lymphocyte activation antigen CD30, Tumor necrosis factor receptor superfamily member 8 (TNFRSF8)
Immunogen Recombinant human full-length TNFRSF8 protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 105-120kDa
Cellular Localization Cell surface
Species Reactivity Human. Others not known.
    

Specificity & Comments

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Hodgkin s lymphoma

Supplied As

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Smith, C.A., et al. 1993. CD30 antigen, a marker for Hodgkin s lymphoma, is a receptor whose ligand defines an emerging family of cytokines with homology to TNF. Cell 73: 1349-1360.
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