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Home >> Antibodies >> CD44v4 (Marker of Tumor Metastasis)

CD44v4 (Marker of Tumor Metastasis)

Recombinant Rabbit Monoclonal Antibody [Clone CD44v4/1700R]

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Catalog No Format Size Price (USD)
960-RBM10-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
960-RBM10-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
960-RBM10-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
960-RBM10-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
960-RBM10-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 960
Human SwissProt P16070
Human Unigene 502328
Human Gene Symbol CD44
Human Chromosome Location 11p13
Synonyms LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8); Epican; Extracellular Matrix Receptor III (ECM III); GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)
Immunogen Recombinant fragment corresponding to the v4 domain of human CD44 (exact sequence is proprietary)
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 80-95kDa
Cellular Localization Cell surface
Species Reactivity Human. Others-not tested.
    

Specificity & Comments

This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Cervix or Tongue Squamous Cell Carcinoma

Supplied As

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Thanakit V, Sampatanukul P, Ruangvejvorachai P, Keelawat S. The association of co-expression of CD44v4/MMP-9 with different nodal status in high-grade breast carcinoma patients. J Med Assoc Thai. 2005 Sep;88 Suppl 4:S30-5.
  2. H. Imam, B. Eriksson & K. Oberg. Expression of CD44 variant isoforms and association to the benign form of endocrine pancreatic tumours. Annals of Oncology 11: 295-300, 2000.
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