|Catalog No||Formulation||Size||Price (USD)|
|821-MSM1-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||129.00|
|821-MSM1-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||329.00|
|821-MSM1-P2||Purified Ab with BSA and Azide at 200ug/ml||200 ug||459.00|
|821-MSM1-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||359.00|
|821-MSM1-P2ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||200 ug||489.00|
|Human Entrez Gene ID||821|
|Human Gene Symbol||CANX|
|Human Chromosome Location||5q35.3|
|Synonyms||Calnexin; CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; P90|
|Immunogen||Recombinant N-terminal fragment of human Calnexin protein (exact sequence is proprietary)|
|Host / Ig Isotype||Mouse / IgG2b, kappa|
|Mol. Weight of Antigen||90kDa|
|Species Reactivity||Human. Does not react with rat. Others not known.|
It recognizes a protein of 90kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.