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Home >> Antibodies >> Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Cytokeratin 7 (Glandular and Transitional Epithelial Marker)

Recombinant Rabbit Monoclonal Antibody [Clone KRT7/1499R]

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Catalog No Format Size Price (USD)
3855-RBM9-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
3855-RBM9-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
3855-RBM9-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
3855-RBM9-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
3855-RBM9-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 3855
Human SwissProt P08729
Human Unigene 411501
Human Gene Symbol KRT7
Human Chromosome Location 12q13.13
Synonyms CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
Immunogen Recombinant full-length human KRT7 protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 55kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others not known.
    

Specificity & Comments

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa cells. Carcinoma of ovary, lung, cervix, or breast

Supplied As

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55
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