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Home >> Antibodies >> Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker)

Recombinant Rabbit Monoclonal Antibody [Clone GPC3/1534R]

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Catalog No Format Size Price (USD)
2719-RBM4-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
2719-RBM4-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
2719-RBM4-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
2719-RBM4-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
2719-RBM4-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 2719
Human SwissProt P51654
Human Unigene 644108
Human Gene Symbol GPC3
Human Chromosome Location Xq26.2
Synonyms DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1
Immunogen Recombinant human full-length GPC3 protein
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 67kDa
Cellular Localization Cytoplasmic
Species Reactivity Human and Rat. Others-not known
    

Specificity & Comments

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with1mM EDTA buffer, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

293T cells or Hepatocellular carcinoma

Supplied As

200ug/ml of Ab purified by Protein A. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Yan, B., et al. 2011. Expression and clinicopathologic significance of glypican 3 in hepatocellular carcinoma. Ann. Diagn. Pathol. 15: 162-169.
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