|Catalog No||Formulation||Size||Price (USD)|
|2315-RBM9-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|2315-RBM9-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|2315-RBM9-P2||Purified Ab with BSA and Azide at 200ug/ml||200 ug||549.00|
|2315-RBM9-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|2315-RBM9-P2ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||200 ug||579.00|
|Human Entrez Gene ID||2315|
|Human Gene Symbol||MLANA|
|Human Chromosome Location||9p24.1|
|Synonyms||Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA|
|Immunogen||Recombinant human full-length MLANA protein|
|Host / Ig Isotype||Rabbit / IgG, kappa|
|Mol. Weight of Antigen||20-22kDa (doublet)|
|Species Reactivity||Human, Mouse and Rat. Others-not tested.|
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Flow Cytometry (0.5-1ug/million cells)
Western Blotting (0.5-1.0ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.