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Home >> Antibodies >> NKX2.2 (Neuroendocrine & Ewing s Sarcoma Marker)

NKX2.2 (Neuroendocrine & Ewing s Sarcoma Marker)

Recombinant Rabbit Monoclonal Antibody [Clone NX2/1422R]

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Catalog No Format Size Price (USD)
4821-RBM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00
4821-RBM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 379.00
4821-RBM2-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 549.00
4821-RBM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 399.00
4821-RBM2-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 579.00
Human Entrez Gene ID 4821
Human SwissProt O95096
Human Unigene 516922
Human Gene Symbol NKX2-2
Human Chromosome Location 20p11.22
Synonyms Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman
Immunogen Recombinant human NKX2.2 protein fragment (exact sequence is proprietary)
Host / Ig Isotype Rabbit / IgG, kappa
Mol. Weight of Antigen 40-50kDa
Cellular Localization Nuclear
Species Reactivity Human. Others not known.
    

Specificity & Comments

Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Pancreas or Ewing s sarcoma

Supplied As

200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Wang, Y.C. et al. (2009) Endocr Relat Cancer 16, 267-79.
  2. Yoshida A et. al. Am J Surg Pathol. 2012;36(7):993-9.
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