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Home >> Antibodies >> Napsin A (Lung Adenocarcinoma Marker)

Napsin A (Lung Adenocarcinoma Marker)

Mouse Monoclonal Antibody [Clone NAPSA/1239]

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Catalog No Format Size Price (USD)
9476-MSM3-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
9476-MSM3-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
9476-MSM3-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
9476-MSM3-P1ABX Purified Ab WITHOUT BSA at 1.0mg/ml 100 ug 359.00
9476-MSM3-P2ABX Purified Ab WITHOUT BSA at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 9476
Human SwissProt O96009
Human Unigene 512843
Human Gene Symbol NAPSA
Human Chromosome Location 19q13.33
Synonyms ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA
Immunogen Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG1, kappa
Mol. Weight of Antigen 37kDa
Cellular Localization Cytoplasmic
Species Reactivity Human. Others-not known.
         

Specificity & Comments

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (1-2ug/ml for 60 minutes at RT)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Lung adenocarcinoma

Supplied As

200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sBishop, JA et. al. Hum Pathol 41: 20-25
  2. Ordonez, NG 2012 Adv Anat Pathol 19: 66-73
  3. Ye, J et. al. Appl Immunhistochem Mol Morphol 19: 313-317
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