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Home >> Antibodies >> PAX8 (Renal Cell Marker)

PAX8 (Renal Cell Marker)

Mouse Monoclonal Antibody [Clone PAX8/1492]

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Catalog No Format Size Price (USD)
7849-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
7849-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
7849-MSM2-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
7849-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
7849-MSM2-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 7849
Human SwissProt Q06710
Human Unigene 469728
Human Gene Symbol PAX8
Human Chromosome Location 2q13
Synonyms Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax-8; Paired domain gene 8; PAX8
Immunogen Recombinant human PAX8 fragment (aa60-261) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2a
Mol. Weight of Antigen 62kDa
Cellular Localization Nuclear
Species Reactivity Human. Others not known.
                   

Specificity & Comments

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

Raji cells. Renal Cell Carcinoma (RCC)

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sTong GX, Yu WM, Beaubier NT, Weeden EM, Hamele-Bena D, Mansukhani MM, O'Toole KM. Expression of PAX8 in normal and neoplastic renal tissues: an immunohistochemical study. Mod Pathol. 2009 Sep;22(9):1218-27.
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