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Home >> Antibodies >> Thrombomodulin / CD141 (Endothelial Cell Marker)

Thrombomodulin / CD141 (Endothelial Cell Marker)

Mouse Monoclonal Antibody [Clone THBD/1591]

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Catalog No Format Size Price (USD)
7056-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
7056-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
7056-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
7056-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
7056-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 7056
Human SwissProt P07204
Human Unigene 2030
Human Gene Symbol THBD
Human Chromosome Location 20p11.2
Synonyms AHUS6; BDCA3; CD141; Fetomodulin; Thbd; THPH12; THRM; Thrombomodulin (TM)
Immunogen Recombinant human Thrombomodulin protein fragment (aa69-194) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen ~100kDa
Cellular Localization Cell Surface & Cytoplasmic
Species Reactivity Human. Others-not known.
                   

Specificity & Comments

It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

Known Applications & Suggested Dilutions

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (0.5-1ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris buffer with1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. sAppleton MA, Attanoos RL, Jasani B. Thrombomodulin as a marker of vascular and lymphatic tumours. Histopathology. 1996 Aug;29(2):153-7.
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