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Home >> Antibodies >> Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)

Topoisomerase II alpha (Proliferation & Drug-Resistance Marker)

Mouse Monoclonal Antibody [Clone TOP2A/1361]

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Catalog No Format Size Price (USD)
7153-MSM1-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 129.00
7153-MSM1-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 329.00
7153-MSM1-P2 Purified Ab with BSA and Azide at 200ug/ml 200 ug 459.00
7153-MSM1-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 359.00
7153-MSM1-P2ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 200 ug 489.00
Human Entrez Gene ID 7153
Human SwissProt P11388
Human Unigene 156346
Human Gene Symbol TOP2A
Human Chromosome Location 17q21.2
Synonyms ATP hydrolyzing DNA topoisomerase II alfa; DNA gyrase; DNA topoisomerase (ATP hydrolyzing); DNA topoisomerase 2 alpha; DNA topoisomerase II 170kD; DNA topoisomerase II alpha; DNA Topoisomerase2; TOP2A; Topoisomerase DNA II alpha 170kDa; TP2A
Immunogen Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)
Host / Ig Isotype Mouse / IgG2b, kappa
Mol. Weight of Antigen 170kDa
Cellular Localization Nuclear
Species Reactivity Human and Mouse. Others not known.
              

Specificity & Comments

It recognizes a 170kDa protein, which is identified as topoisomerase II.  shows no cross-reaction with Topoisomerase II or I. Topo II plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II is also implicated in drug resistance of tumor cells.

Known Applications & Suggested Dilutions

Flow Cytometry (0.5-1ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blotting (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 min at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Positive Control

HeLa cells or Tonsil

Supplied As

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Storage and Stability

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Key References

  1. Chung, T.D., et al. 1989. Characterization and immunological identification of cDNA clones encoding two human DNA topoisomerase II isozymes. Proc. Natl. Acad. Sci USA 86: 9431-9435.
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