|Catalog No||Formulation||Size||Price (USD)|
|7490-RBM4-P0||Purified Ab with BSA and Azide at 200ug/ml||20 ug||199.00|
|7490-RBM4-P1||Purified Ab with BSA and Azide at 200ug/ml||100 ug||379.00|
|7490-RBM4-P2||Purified Ab with BSA and Azide at 200ug/ml||200 ug||549.00|
|7490-RBM4-P1ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||100 ug||399.00|
|7490-RBM4-P2ABX||Purified Ab WITHOUT BSA and Azide at 1.0mg/ml||200 ug||579.00|
|Human Entrez Gene ID||7490|
|Human Gene Symbol||WT1|
|Human Chromosome Location||11p13|
|Synonyms||WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1|
|Immunogen||Recombinant human full-length WT1 protein|
|Host / Ig Isotype||Rabbit / IgG, kappa|
|Mol. Weight of Antigen||47-55kDa|
|Cellular Localization||Nuclear & Cytoplasmic|
|Species Reactivity||Human. Others-not known|
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Flow Cytometry (0.5-1ug/million cells)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
K562 cells. Wilm s Tumor, mesothelioma or fetal kidney.
200ug/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
This antibody is available for research use only and is not approved for use in diagnosis.
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.