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Aromatase / Cytochrome P450 (CYP19A1) Antibody [CYP19A1/4257]

In Stock
Catalog Number Formulation Size Price
1588-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1588-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1588-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Aromatase / Cytochrome P450 (CYP19A1) Antibody

SDS-PAGE Analysis of Purified Aromatase / CYP450 Mouse Monoclonal Antibody (CYP19A1/4257). Confirmation of Integrity and Purity of Antibody.

Aromatase / Cytochrome P450 (CYP19A1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human placenta stained with Aromatase / CYP450 Mouse Monoclonal Antibody (CYP19A1/4257).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CYP450 Aromatase is part of the CYP19A1 family involved in the aromatization of androgens to estrogens, a highly conserved mechanism amongst mammals. Clone CYP19A1/4257 recognizes a conserved epitope on the CYP450 aromatase, allowing for detection of aromatase levels using various analysis methods.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease, Mitochondria Marker
Isotype / Light Chain
IgG2a /
Cellular Localization
Endoplasmic reticulum, Microsome membrane
Gene Name
CYP19A1
Positive Control
Human normal placenta.
Immunogen
Synthetic peptide corresponding to aa376-390 of human aromatase (KALEDDVIGYPVKK)
Alternate Names
Aromatase, CYPXIX, Cytochrome P-450AROM, Cytochrome P450 19A1, Estrogen synthase, ARO1, CYAR, CYP19

Database Links

Entrez Gene ID
1588
SwissProt
P11511

Additional Information

Clone
CYP19A1/4257
Chromosome Location
2q32.2
Mol. Weight of Antigen
55kDa

Related Antibodies

View All CYP19A1 Antibodies

Functions

  • A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen.Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Key References

  • Turner, KJ, et al. (2002) Development and Validation of a New Monoclonal Antibody to Mammalian Aromatase. Journal of Endocrinology.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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