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NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Antibody [SPM347]

In Stock
Catalog Number Formulation Size Price
2026-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2026-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2026-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).

Formalin-fixed, paraffin-embedded human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).

SDS-PAGE Analysis of Purified NSE gamma Monoclonal Antibody (SPM347). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified NSE gamma Monoclonal Antibody (SPM347). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Neuroscience, Mast Cell Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
ENO2
Positive Control
Cerebellum or Pheochromocytoma., HeLa or Y79 cells. Pancreas, HePG2, SH-SY-5Y
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433; exact sequence is proprietary)
Alternate Names
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE

Database Links

Entrez Gene ID
2026
SwissProt
P09104

Additional Information

Clone
SPM347
Chromosome Location
12p13
Mol. Weight of Antigen
~50kDa

Related Antibodies

View All ENO2 Antibodies

Functions

  • Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Key References

  • Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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