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HLA-DP (MHC II) Antibody [SPM421]

In Stock
Catalog Number Formulation Size Price
3115-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3115-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3115-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
HLA-DP (MHC II) Antibody

Flow Cytometric Analysis of Human Raji cells using HLA-DP (MHC II) Monoclonal Antibody (SPM421) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Immuno Oncology, Immunology, Cytokine Signaling, Dendritic Cell Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, trans-Golgi network membrane
Gene Name
HLA-DPB1
Positive Control
Raji cells. Tonsil or lymph node.
Immunogen
Non-T, non-B human acute lymphoblastic leukemia REH6 cell line
Alternate Names
HLA class II histocompatibility antigen, DP beta 1 chain, HLA class II histocompatibility antigen, DP(W4) beta chain, MHC class II antigen DPB1, HLA class II histocompatibility antigen DP alpha 1 chain; HLA DP1A; HLA SB alpha chain; HLADP; HLADPA1; HLASB; HLASB histocompatibility type; Human MHC class II HLA SB alpha; Major histocompatibility complex class II DP alpha 1; MHC class II DP3 alpha; MHC class II DPA1; PLT1; Primed lymphocyte test 1

Database Links

Entrez Gene ID
3115
SwissProt
P04440

Additional Information

Clone
SPM421
Chromosome Location
6p21.3
Mol. Weight of Antigen
33-35kDa

Related Antibodies

View All HLA-DPB1 Antibodies

Functions

  • Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Key References

  • Horejsi V, et. al. Tissue Antigens, 1988, 32(1):6-11.
  • Polakova K; Chorvath B; Sedlak J; Duraj J; Matoska J; Karpatova M. Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line. Neoplasma, 1985, 32(6):641-8.
  • Babusikova O; Ujhazy P; Hrivnakova A; Chorvath B; Polakova K. Studies on the distribution of the antigens detected by some newly prepared monoclonal antibodies in normal hemopoietic and leukemic cells. Neoplasma, 1985, 32(6):657-62.
  • Neoplasma 34(4), 417- 425, (1987).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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