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FOXA1 / HNF3A Antibody [FOXA1/1518]

In Stock
Catalog Number Formulation Size Price
3169-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3169-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3169-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1518).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with FOXA1 Monoclonal Antibody (FOXA1/1518).

Formalin-fixed, paraffin-embedded Rat Colon stained with FOXA1 Monoclonal Antibody (FOXA1/1518).

Formalin-fixed, paraffin-embedded Rat Colon stained with FOXA1 Monoclonal Antibody (FOXA1/1518).

Western Blot Analysis of human HeLa cell lysate using FOXA1 Monoclonal Antibody (FOXA1/1518).

Western Blot Analysis of human HeLa cell lysate using FOXA1 Monoclonal Antibody (FOXA1/1518).

SDS-PAGE Analysis Purified FOXA1 Monoclonal Antibody (FOXA1/1518). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis Purified FOXA1 Monoclonal Antibody (FOXA1/1518). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of PFA fixed MCF-7 cells using FOXA1 Monoclonal Antibody (FOXA1/1518); followed by goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 Is Control (Red).

Flow Cytometric Analysis of PFA fixed MCF-7 cells using FOXA1 Monoclonal Antibody (FOXA1/1518); followed by goat anti-mouse IgG-CF488 (Blue); Goat anti-mouse IgG-CF488 Is Control (Red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled withFOXA1 Monoclonal Antibody (FOXA1/1518)followed by goat anti- mouse IgG-CF488. Counterstain is Phalloiden (Red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled withFOXA1 Monoclonal Antibody (FOXA1/1518)followed by goat anti- mouse IgG-CF488. Counterstain is Phalloiden (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Research Areas
Cancer, Developmental Biology, Nuclear Marker, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus
Gene Name
FOXA1
Positive Control
Bladder, Colon and Lung and Breast., HeLa, HepG-2 or MCF-7 cells. Liver, Pancreas, prostate
Immunogen
Recombinant fragment of human FOXA1 protein (aa372-472) (exact sequence is proprietary)
Alternate Names
Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, Transcription factor 3A, Forkhead box protein A1 (FOXA1); Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A); Transcription factor 3A (TCF-3A or TCF3A)

Database Links

Entrez Gene ID
3169
SwissProt
P55317

Additional Information

Clone
FOXA1/1518
Chromosome Location
14q21.1
Mol. Weight of Antigen
49kDa

Related Antibodies

View All FOXA1 Antibodies

Functions

  • Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.

Key References

  • Andre Albergaria, Joana Paredes, Barbara Sousa, Fernanda Milanezi, Vitor Carneiro, Joana Bastos, Sandra Costa, Daniella Vieira, Nair Lopes, Eric W Lam, Nuno Lunet and Fernando Schmitt. Expression of FOXA1 and GATA-3 in breast cancer: the prognostic significance in hormone receptor-negative tumours. Breast Cancer Research 2009, 11:R40.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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