Free Shipping in the U.S. for orders over $1000.  Shop Now>>

Printable Datasheet

Recombinant Interferon gamma (IFNG) Antibody [IFNG/3996R]

In Stock
Catalog Number Formulation Size Price
3458-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3458-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3458-RBM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human kidney stained with Interferon gamma Recombinant Rabbit Monoclonal Antibody (IFNG/3996R).

Formalin-fixed, paraffin-embedded human kidney stained with Interferon gamma Recombinant Rabbit Monoclonal Antibody (IFNG/3996R).

Formalin-fixed, paraffin-embedded human kidney stained with Interferon gamma Recombinant Rabbit Monoclonal Antibody (IFNG/3996R).

Formalin-fixed, paraffin-embedded human kidney stained with Interferon gamma Recombinant Rabbit Monoclonal Antibody (IFNG/3996R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-纬 and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-纬. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-伪/尾 (type I interferon).

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Colon Cancer, Complement System, Cytokine Signaling, Hematopoietic Stem Cells, Neuroinflammation, PD-1 blockade immunotherapy, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Secreted
Gene Name
IFNG
Positive Control
PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.
Immunogen
Recombinant full-length human IFNG protein
Alternate Names
Interferon gamma, Immune interferon, AMCF1; Beta thromboglobulin like protein; CXC chemokine ligand 8 (CXCL8); Emoctakin; Granulocyte chemotactic protein 1 (GCP1); IFG; IFI; IFN Immune; Interleukin 8; LECT; LUCT; Lymphocyte derived neutrophil activating factor; LYNAP; Macrophage Activating Factor (MAF); Monocyte derived neutrophil activating protein (MONAP); Monocyte derived neutrophil chemotactic factor (MDNCF); Neutrophil activating factor (NAF); Neutrophil activating peptide 1 (NAP1); Neutrophil activating protein 1 (NAP1); SCYB8; T cell chemotactic factor; T Cell Interferon

Database Links

Entrez Gene ID
3458
SwissProt
P01579

Additional Information

Clone
IFNG/3996R
Chromosome Location
12q14
Mol. Weight of Antigen
20-25kDa

Related Antibodies

View All IFNG Antibodies

Functions

  • Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

Key References

  • Vilcek J. Cytokine Growth Factor Rev 1997,8(4):239
  • Farrar MA and Schreiber RD. Annu Rev Immunol 1993, 11:571-611
  • Vilcek J., et al. Methods Enzymol 1986,119:48-54

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Interferon gamma (IFNG) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales