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Prostate Specific Antigen (PSA) Antibody [A67-B/E3 + 1A7]

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with PSA Monoclonal Antibody (A67-B/E3 + 1A7).

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular, Signal Transduction

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Secreted

Gene Name

KLK3

Positive Control

PC12 cells. Normal prostate or prostate carcinoma.

Immunogen

PSA (p30) from human sperm plasma

Alternate Names

Prostate-specific antigen, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Database Links

Entrez Gene ID

354

SwissProt

P07288

Additional Information

Clone

A67-B/E3 + 1A7

Chromosome Location

19q13.33

Mol. Weight of Antigen

33-34kDa

Functions

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Key References

ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999).
Huang et al. Prostate-specific antigen immunosensing based on mixed self-assembled monolayers, camel antibodies and colloidal gold enhanced sandwich assays. Biosensors and Bioelectronics. 21/3; 483-490 (2005).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Prostate Specific Antigen (PSA) Antibody [A67-B/E3 + 1A7]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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Recombinant Prostate Specific Antigen (PSA) Antibody [KLK3/7128R]

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