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Recombinant Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker) Antibody [KRT15/2103R]

In Stock
Catalog Number Formulation Size Price
3866-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3866-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3866-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R).

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R).

Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R).

Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R).

SDS-PAGE Analysis of Purified KRT15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified KRT15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of HeLa cells. KRT15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of HeLa cells. KRT15 Rabbit Recombinant Monoclonal Antibody (KRT15/2103R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. KRT15 is downregulated in activated keratinocytes. It is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunohistochemistry
Species Reactivity
Cow, Human, Rat
Research Areas
Autophagy, Developmental Biology, Basal Cell Marker
Isotype / Light Chain
IgG / Kappa
Gene Name
KRT15
Positive Control
A431 cells. Skin or Colon., HeLa
Immunogen
Recombinant human full-length KRT15 protein
Alternate Names
Keratin, type I cytoskeletal 15, Cytokeratin-15, Keratin-15, CK15; Cytokeratin 15; K1CO; Ka15; Keratin 15 basic; Keratin 15 beta; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15

Database Links

Entrez Gene ID
3866
SwissProt
P19012

Additional Information

Clone
KRT15/2103R
Chromosome Location
17q21.2
Mol. Weight of Antigen
52kDa

Related Antibodies

View All KRT15 Antibodies

Key References

  • Yu-Hong Shen, et al. Asian Pac J Cancer Prev, 17 (9), 4217-4222.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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