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NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker) Antibody [SPM299]

In Stock
Catalog Number Formulation Size Price
4804-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4804-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4804-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (SPM299).

Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (SPM299).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Baboon, Cat, Ferret, Human, Monkey, Rabbit
Research Areas
Neuroscience, AKT Signaling, Mesenchymal Stem Cell Differentiation, Nuclear Marker, Signal Transduction, Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cell projection, Dendritic spine, Growth cone, Perikaryon
Gene Name
NGFR
Positive Control
Neurofibroma. Soma and axons of sensory neurons and ganglionic satellite cells. Melanomas., Neuronal axons, Schwann cells and perineural cells of peripheral nerves or tumors of nerve sheath differentiation e. G. Schwannoma
Immunogen
NGFR from A875 melanoma cells
Alternate Names
Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, p75 ICD, CD271; Gp80-LNGFR; Low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Nerve growth factor receptor (NGFR); TNFR superfamily member 16; p75 ICD; p75 Neurotrophin receptor; Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)

Database Links

Entrez Gene ID
4804
SwissProt
P08138

Additional Information

Clone
SPM299
Chromosome Location
17q21-22
Mol. Weight of Antigen
75kDa

Related Antibodies

View All NGFR Antibodies

Functions

  • Low affinity receptor which can bind to NGF, BDNF, NTF3, and NTF4. Forms a heterodimeric receptor with SORCS2 that binds the precursor forms of NGF, BDNF and NTF3 with high affinity, and has much lower affinity for mature NGF and BDNF (PubMed:24908487). Plays an important role in differentiation and survival of specific neuronal populations during development (By similarity). Can mediate cell survival as well as cell death of neural cells. Plays a role in the inactivation of RHOA (PubMed:26646181). Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCmgetaN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver (PubMed:23785138).

Key References

  • Marano N, et. al. Journal of Neurochemistry, 1987, 48:225-32.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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