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CD31 / PECAM-1 (Endothelial Cell Marker) Antibody [C31.10]

In Stock
Catalog Number Formulation Size Price
5175-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5175-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5175-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD31 Antibody in Immunohistochemistry (IHC (P))

CD31 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Tonsil stained with CD31 Mouse Monoclonal Antibody (C31.10). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

CD31 Antibody in Immunohistochemistry (IHC (P))

CD31 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31 Mouse Monoclonal Antibody (C31.10).

CD31 Antibody in Western Blot (WB)

CD31 Antibody in Western Blot (WB)

Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.10).

CD31 Antibody in Flow Cytometry (FC)

CD31 Antibody in Flow Cytometry (FC)

Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled CD31 Mouse Monoclonal Antibody (C31.10).

CD31 Antibody in SDS-PAGE

CD31 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD31 Mouse Monoclonal Antibody (C31.10). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Angiogenesis, Endothelial Cell Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Membrane raft
Gene Name
PECAM-1
Positive Control
Human tonsil. Jurkat cells.
Immunogen
Recombinant human CD31 protein
Alternate Names
Platelet endothelial cell adhesion molecule, EndoCAM, GPIIA', PECA1, EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; PECAM1; GPIIA'

Database Links

Entrez Gene ID
5175
SwissProt
P16284

Additional Information

Clone
C31.10
Chromosome Location
17q23.3
Mol. Weight of Antigen
~100kDa (endothelium) and ~130kDa (platelets)'

Related Antibodies

Functions

  • Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).
  • Does not protect against apoptosis.

Key References

  • Stockinger H, Gadd S J, Eher R, et al. Molecular characterization and functional analysis of the leukocyte surface protein CD31. Journal of Immunology. 145 (11): 3889-3897 (1990).
  • Stockinger H, Schreiber W, Majdic O, et al. Phenotype of human T cells expressing CD31, a molecule of the immunoglobulin supergene family. Immunology. 75 (1): 53-58 (1992)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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