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Cyclin D1 (G1-Cyclin & Mantle Cell Marker) Antibody [DCS-6]

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Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Mantle Cell Lymphoma stained with Cyclin D1 Mouse Monoclonal Antibody (DCS-6).

Cyclin D1 Antibody in Flow Cytometry (FC)

Flow Cytometry of human Cyclin D1 on Jurkat cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Mouse Monoclonal Antibody (DCS-6).

Cyclin D1 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of human Cyclin D1 on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Cyclin D1 Monoclonal Antibody (DCS-6).

Cyclin D1 Antibody in Western Blot (WB)

Western Blot Analysis of Cyclin D1 in (1) C2C12, (2) HepG2, & (3) NIH3T3 cell lysate Cyclin D1 Mouse Monoclonal Antibody (DCS-6).

Cyclin D1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Cyclin D1 Mouse Monoclonal Antibody (DCS-6). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Western Blot (WB)

2-4ug/ml

Western Blot Protocol

Summary

Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40% of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human

Research Areas

Breast Cancer, Cancer, Cardiovascular, Immunology, AKT Signaling, Bladder Cancer, Colon Cancer, Cytokine Signaling, Infectious Disease, Lung Cancer, Nuclear Marker, Signal Transduction, Transcription Factors

Isotype / Light Chain

IgG2a / Kappa

Cellular Localization

Cytoplasm, Nucleus, Nucleus membrane

Gene Name

CCND1

Positive Control

T47D, Jurkat or MCF-7 cells. Mantle cell lymphoma.

Immunogen

Human full length recombinant cyclin D1 protein

Alternate Names

G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene

Database Links

Entrez Gene ID

595

SwissProt

P24385

Additional Information

Clone

DCS-6

Chromosome Location

11q13.2

Mol. Weight of Antigen

36kDa

Functions

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162, PubMed:33854235). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Hypophosphorylates RB1 in early G(1) phase (PubMed:1833066, PubMed:1827756, PubMed:8114739, PubMed:8302605, PubMed:19412162). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1833066, PubMed:1827756, PubMed:8302605, PubMed:19412162). Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).

Key References

Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7.
Lukas J, et. al. Oncogene, 1994, 9(3):707-18.
Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Cyclin D1 (G1-Cyclin & Mantle Cell Marker) Antibody [DCS-6]

Cyclin D1 Antibody in Immunohistochemistry (IHC (P))

Cyclin D1 Antibody in Flow Cytometry (FC)

Cyclin D1 Antibody in Flow Cytometry (FC)

Cyclin D1 Antibody in Western Blot (WB)

Cyclin D1 Antibody in SDS-PAGE

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

Recombinant Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) Antibody [rCCND1/4752]

Recombinant Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker) Antibody [CCND1/3370R]