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NF-kB p65 / RELA Antibody [PCRP-RELA-1E3]

In Stock
Catalog Number Formulation Size Price
5970-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5970-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5970-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Flow Cytometric Analysis of PFA-fixed K562 cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed K562 cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells using NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunofluorescence Analysis of PFA-fixed MCF-7 cells using NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometric Analysis of PFA-fixed HeLa cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed HeLa cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed U87 cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed U87 cells. NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Analysis of Protein Array containing more than 19,000 full-length human proteins using NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE Analysis.Purified NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis.Purified NF-kB p65 / RELA Mouse Monoclonal Antibody (PCRP-RELA-1E3). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml

Summary

Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor N B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp B sequence in the immunoglobulin light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NF B is activated and NF B is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular, Developmental Biology, Immunology, Cytokine Signaling, Infectious Disease, Lung Cancer, MAPK Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG2b /
Cellular Localization
Cytoplasm, Nucleus
Gene Name
RELA
Positive Control
HeLa, K562 or MCF-7 cells., U87
Immunogen
Recombinant full-length human RELA protein
Alternate Names
Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Nuclear Factor NF Kappa B p65 Subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; Transcription factor p65; V rel avian reticuloendotheliosis viral oncogene homolog A; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65; NFKB3

Database Links

Entrez Gene ID
5970
SwissProt
Q04206

Additional Information

Clone
PCRP-RELA-1E3
Chromosome Location
11q13.1
Mol. Weight of Antigen
65kDa

Related Antibodies

View All RELA Antibodies

Functions

  • NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Key References

  • Young, J.L., et al. 1969. Secretion of sodium and potassium by the parotid gland in essential hypertension. South. Med. J. 62: 468-470.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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