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Bcl-X (Apoptosis Marker) Antibody [BX006]

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Western Blot Analysis of Jurkat cell lysate Bcl-x Mouse Monoclonal Antibody (BX006).

Formalin-fixed, paraffin-embedded Hodgkin's lymphoma stained with Bcl-x Mouse Monoclonal Antibody (BX006).

SDS-PAGE Analysis of Purified Bcl-x Mouse Monoclonal Antibody (BX006). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Western Blot (WB)

2-4ug/ml

Western Blot Protocol

Summary

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunohistochemistry, Western Blot

Species Reactivity

Human, Mouse, Pig, Rat

Research Areas

Apoptosis, Autophagy, Cardiovascular, Immunology, Colon Cancer, Cytokine Signaling, Infectious Disease, Lung Cancer, Mitochondria Marker, Signal Transduction

Isotype / Light Chain

IgG2a / Kappa

Cellular Localization

Centrosome, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Cytosol, Microtubule organizing center, Mitochondrion inner membrane, Mitochondrion matrix, Mitochondrion outer membrane, Nucleus membrane, Secretory vesicle, Synaptic vesicle membrane

Gene Name

BCL2L1

Positive Control

HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin's lymphoma., Jurkat, K562

Immunogen

A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.

Alternate Names

Bcl-2-like protein 1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl-X, B cell lymphoma 2 like, BCL XL/S, Bcl-2-like protein 1, Bcl2-L-1, BCL2L, Bcl2l1, BCLX, BclXL, BclXs, Protein phosphatase 1 regulatory subunit 52 (PPP1R52)

Database Links

Entrez Gene ID

598

SwissProt

Q07817

Additional Information

Clone

BX006

Chromosome Location

20q11.21

Mol. Weight of Antigen

27kDa

Functions

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.
Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
Isoform Bcl-X(S) promotes apoptosis.

Key References

Hsu YT, et. al. Journal of Biological Chemistry, 1997, 272(21):13829-34.
Hsu YT, et. al. PNAS, 1997, 94(8):3668-72.
Wolter KG, et. al. Journal of Cell Biology, 1997, 139(5):1281-92.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Bcl-X (Apoptosis Marker) Antibody [BX006]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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