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Printable Datasheet

Spectrin beta I (SPTB) Antibody [RG/26]

In Stock
Catalog Number Formulation Size Price
6710-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
6710-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
6710-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Spectrin beta I (SPTB) Antibody

SDS-PAGE Analysis of Purified Spectrin beta III Mouse Monoclonal Antibody (RG/26). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Mouse, Rat
Research Areas
Developmental Biology, Signal Transduction
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell cortex, Cytoplasm, Cytoskeleton
Gene Name
SPTBN2
Positive Control
Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).
Immunogen
Red cell membranes
Alternate Names
Spectrin beta chain, non-erythrocytic 2, Beta-III spectrin, Spinocerebellar ataxia 5 protein, beta-I spectrin; EL3; erythrocyte; HS2; SPTB1; HSPTB1; membrane cytoskeletal protein; spectrin beta (erythrocytic)

Database Links

Entrez Gene ID
6710
SwissProt
O15020

Additional Information

Clone
RG/26
Chromosome Location
14q23.3
Mol. Weight of Antigen
246kDa

Related Antibodies

View All SPTBN2 Antibodies

Functions

  • Probably plays an important role in neuronal membrane skeleton.

Key References

  • Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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