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TIA1 (T-Cell-Restricted Intracellular Antigen-1) Antibody [TIA1/1313]

In Stock
Catalog Number Formulation Size Price
7072-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7072-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7072-MSM1-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
TIA1 (T-Cell-Restricted Intracellular Antigen-1) Antibody

SDS-PAGE Analysis of Purified TIA1 Monoclonal Antibody (TIA1/1313). Confirmation of Purity and Integrity of Antibody.

TIA1 (T-Cell-Restricted Intracellular Antigen-1) Antibody - Image 2

Western Blot of HepG2, PC3 and HeLa cell lysates with TIA1 Monoclonal Antibody (TIA1/1313).

TIA1 (T-Cell-Restricted Intracellular Antigen-1) Antibody - Image 3

Immunofluorescence Analysis of PFA-fixed HeLa cells using TIA1 Mouse Monoclonal Antibody (TIA1/1313) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.

Applications & Dilutions

Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

TIA-1 (T-cell intracytoplasmic antigen) is a cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. TIA-1 is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15kDa protein thought to be derived from the carboxyl terminus of the 40kDa product by proteolytic processing. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Nuclear Marker, Signal Transduction
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Nucleus, Stress granule
Gene Name
TIA1
Positive Control
HePG2, PC3 or HeLa cells. Human tonsil.
Immunogen
Recombinant human TIA1 fragment of 102 amino acid residues (aa279-380)
Alternate Names
Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, p40-TIA-1, Cytotoxic granule associated RNA binding protein 1; mTIA-1; Nucleolysin TIA1 isoform p40; p40-TIA-1; RNA binding protein TIA1; T-cell-restricted intracellular antigen-1 (TIA1); TIA-1; TIA1; TIA1 cytotoxic granule associated RNA binding protein; TIAL1; TIAR; WDM

Database Links

Entrez Gene ID
7072
SwissProt
P31483

Additional Information

Clone
TIA1/1313
Chromosome Location
2p13
Mol. Weight of Antigen
40kDa

Related Antibodies

View All TIA1 Antibodies

Functions

  • RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences (PubMed:8576255, PubMed:11106748, PubMed:12486009, PubMed:17488725). Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing (PubMed:11106748, PubMed:8576255). Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1 (By similarity). Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence (PubMed:11106748). By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP (PubMed:11106748, PubMed:12486009, PubMed:17488725). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIAR mRNA (By similarity). Acts as a modulator of alternative splicing for the apoptotic FAS receptor, thereby promoting apoptosis (PubMed:11106748, PubMed:1934064, PubMed:17488725). Binds to the 5' splice site region of FAS intron 5 to promote accumulation of transcripts that include exon 6 at the expense of transcripts in which exon 6 is skipped, thereby leading to the transcription of a membrane-bound apoptotic FAS receptor, which promotes apoptosis (PubMed:11106748, PubMed:1934064, PubMed:17488725). Binds to a conserved AU-rich cis element in COL2A1 intron 2 and modulates alternative splicing of COL2A1 exon 2 (PubMed:17580305). Also binds to the equivalent AT-rich element in COL2A1 genomic DNA, and may thereby be involved in the regulation of transcription (PubMed:17580305). Binds specifically to a polypyrimidine-rich controlling element (PCE) located between the weak 5' splice site and the intronic splicing silencer of CFTR mRNA to promote exon 9 inclusion, thereby antagonizing PTB1 and its role in exon skipping of CFTR exon 9 (PubMed:14966131). Involved in the repression of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs), including target ARE-bearing mRNAs encoding TNF and PTGS2 (By similarity). Also participates in the cellular response to environmental stress, by acting downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SGs), leading to stress-induced translational arrest (PubMed:10613902). Formation and recruitment to SGs is regulated by Zn(2+) (By similarity). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed:1934064).
  • Displays enhanced splicing regulatory activity compared with TIA isoform Long.

Key References

  • Anderson, P. 1995. TIA-1: structural and functional studies on a new class of cytolytic effector molecule. Curr. Topics Microbiol. Immunol. 198: 131-143.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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