Free Shipping in the U.S. for orders over $1000.  Shop Now>>

TLE1 (Synovial Sarcoma Marker) Antibody [TLE1/2051]

In Stock
Catalog Number Formulation Size Price
7088-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7088-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7088-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.
Immunogen
Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
Alternate Names
Transducin-like enhancer protein 1, E(Sp1) homolog, Enhancer of split groucho-like protein 1, Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
TLE1/2051
Chromosome Location
9q21.32
Mol. Weight of Antigen
83kDa

Functions

  • Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

Key References

  • Jagdis A, et al. Prospective evaluation of TLE1 as a diagnostic immunohistochemical marker in synovial sarcoma. Am J Surg Pathol. 2009; 33:1743-51.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “TLE1 (Synovial Sarcoma Marker) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK