Thymidylate Synthase (5-FU Resistance Marker) Antibody [TS106 + TMS715]

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Thymidylate Synthase (5-FU Resistance Marker) Antibody

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Monoclonal Antibody (TS106 + TMS715).

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cytoplasm, Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix, Nucleus

Gene Name

TYMS

Positive Control

5-FU-resistant colon carcinoma cell lines (NCI H630R10, MCF-Ad5 and MCF-Ad10. Testicular carcinomas., NCI H630R1); 5-FU-resistant breast cancer cell lines

Immunogen

Recombinant human thymidylate synthase (TS106 & TMS715)

Alternate Names

Thymidylate synthase, dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Database Links

Entrez Gene ID

7298

SwissProt

P04818

Additional Information

Clone

TS106 + TMS715

Chromosome Location

18p11.32

Mol. Weight of Antigen

36kDa

Functions

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Key References

Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13.
Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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