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Villin (GI-Mucosal & Urogenital Brush Border Marker) Antibody [VIL1/1325]

In Stock
Catalog Number Formulation Size Price
7429-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7429-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7429-MSM2-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Rectum stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

Formalin-fixed, paraffin-embedded human Rectum stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

Formalin-fixed, paraffin-embedded human Colon stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

Formalin-fixed, paraffin-embedded human Colon stained with Villin Mouse Monoclonal Antibody (VIL1/1325).

Western Blot of A549, HepG2 & HCT116 cell lysates Villin Mouse Monoclonal Antibody (VIL1/1325).

Western Blot of A549, HepG2 & HCT116 cell lysates Villin Mouse Monoclonal Antibody (VIL1/1325).

SDS-PAGE Analysis of Purified Villin Mouse Monoclonal Antibody (VIL1/1325). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Villin Mouse Monoclonal Antibody (VIL1/1325). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell projection, Cytoplasm, Cytoskeleton, Filopodium, Filopodium tip, Lamellipodium, Microvillus, Ruffle
Gene Name
VIL1
Positive Control
A549, HepG2 and HCT116 cells. Colon or Rectum.
Immunogen
Recombinant human Villin fragment (around aa179-311) (exact sequence is proprietary))
Alternate Names
Villin-1, VIL1; Villin-1; Villin1

Database Links

Entrez Gene ID
7429
SwissProt
P09327

Additional Information

Clone
VIL1/1325
Chromosome Location
2q35
Mol. Weight of Antigen
93kDa

Related Antibodies

View All VIL1 Antibodies

Functions

  • Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.

Key References

  • West BA, Isaac CA, Carboni JM, Morrow JS, Mooseker MS, Barwick KW. Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms. Gastroenterology. 1988;94:343-52.
  • Bacchi CE, Gown AM. Distribution and pattern of expression of villin, a gastrointestinal-associated cytoskeletal protein, in human carcinomas: a study employing paraffin-embedded tissue. Lab Invest. 1991;64:418-24.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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