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Recombinant p40 (Squamous, Basal & Myoepithelial Cell Marker) Antibody [P40/4396R]

In Stock
Catalog Number Formulation Size Price
8626-RBM14-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
8626-RBM14-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
8626-RBM14-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Formalin-fixed, paraffin-embedded human prostate stained with p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R).

Flow cytometric analysis of PFA-fixed HeLa cells. p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow cytometric analysis of PFA-fixed HeLa cells. p40 Recombinant Rabbit Monoclonal Antibody (P40/4396R) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

p40 antibody (antibody recognizing Np63 only) is an isoform of p63, is thought to function as a stem cell factor, responsible for maintaining cells in an uncommitted state with regenerative potential a role that may be recapitulated in tumors derived from these cells. p40 is normally expressed in the basal or progenitor cell layer of stratified epithelia (eg. squamous, urothelial, bronchial), basal cells of some glandular epithelia (eg. prostate), as well as myoepithelial cells of breast and salivary glands, trophoblasts and thymic epithelial cells. In tumor tissues, p40 expression is specific for squamous cell carcinoma. p40 is equivalent to p63 in sensitivity for lung squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. p40 appears to be a more reliable marker for squamous cell carcinoma.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Flow Cytometry, Immunohistochemistry
Species Reactivity
Human
Research Areas
Basal Cell Marker, Nuclear Marker, Stem Cell Differentiation, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus
Gene Name
TP63
Positive Control
HEK293, lung or bladder squamous cell carcinoma tissue., MDA-MB453 or MCF7 cells. Human prostate
Immunogen
Recombinant fragment (around aa1-100) of human p40 protein (exact sequence is proprietary)
Alternate Names
Tumor protein 63, Chronic ulcerative stomatitis protein, Keratinocyte transcription factor KET, Transformation-related protein 63, Tumor protein p73-like, p40, p51, Amplified in squamous cell carcinoma (AIS); Chronic ulcerative stomatitis protein (CUSP); EEC3; TP73L; LMS; TP63; Keratinocyte transcription factor KET; NBP; P51/P63; p53 like transcription factor; RHS; SHFM4; TAp63alpha; TP53CP; TP53L; Transformation-related protein 63; Trp53rp1; Trp6;3; Tumor protein p53-like; tumor protein p73-like

Database Links

Entrez Gene ID
8626
SwissProt
Q9H3D4

Additional Information

Clone
P40/4396R
Chromosome Location
3q28
Mol. Weight of Antigen
63kDa

Related Antibodies

View All TP63 Antibodies

Functions

  • Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter.

Key References

  • Signoretti S, et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol. 2000; 157(6):1769-75.
  • Terry J, et al. Optimal immunohistochemical markers for distinguishing lung adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg Pathol. 2010; 34(12):1805-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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