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Recombinant CD6 (Negative Marker of T-regulatory Cells) Antibody [C6/2884R]

In Stock
Catalog Number Formulation Size Price
923-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
923-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
923-RBM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R).

SDS-PAGE Analysis of Purified CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of MOLT-4 cells. CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometric Analysis of MOLT-4 cells. CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD6
Positive Control
CCRF-CEM, Jurkat cells, Molt-4, Tonsil.
Immunogen
Human recombinant full-length CD6 protein
Alternate Names
T-cell differentiation antigen CD6, T12, TP120, T12; TP120

Database Links

Entrez Gene ID
923
SwissProt
P30203

Additional Information

Clone
C6/2884R
Chromosome Location
11q12.2
Mol. Weight of Antigen
90-130kDa

Related Antibodies

View All CD6 Antibodies

Functions

  • Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24945728, PubMed:24584089). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).

Key References

  • Bazil, V et. al. 1989. Folia. Biol. 35:289-297.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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