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CD86 (Dendritic Cells Maturation Marker) Antibody [BU63]

In Stock
Best Seller
Catalog Number Formulation Size Price
942-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
942-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
942-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD86 Antibody in Immunofluorescence (IF)

CD86 Antibody in Immunofluorescence (IF)

Immunofluorescence staining of PFA-fixed Ramos cells using CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

CD86 Antibody in Flow Cytometry (FC)

CD86 Antibody in Flow Cytometry (FC)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMCs). Red: PE-labeled CD86 Mouse Monoclonal Antibody (BU63); Green: Isotype Control.

CD86 Antibody in SDS-PAGE

CD86 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD86 Mouse Monoclonal Antibody (BU63). Confirmation of Integrity and Purity of Antibody.

CD86 Antibody in Flow Cytometry (FC)

CD86 Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed Ramos cells. CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, B Cell Markers, Cytokine Signaling, Dendritic Cell Marker, Hematopoietic Stem Cells, Infectious Disease, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cell surface
Gene Name
CD86
Positive Control
293T or Jurkat cells. Monocytes and dendritic cells.
Immunogen
ARH-77 (B-lymphoblastoid cell line)
Alternate Names
T-lymphocyte activation antigen CD86, Activation B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1, Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell co-stimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2

Database Links

Entrez Gene ID
942
SwissProt
P42081

Additional Information

Clone
BU63
Chromosome Location
3q13.33
Mol. Weight of Antigen
70kDa

Related Antibodies

View All CD86 Antibodies

Functions

  • Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
  • Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Key References

  • Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  • Mauri D et. al. J Immunol. 1995;155(1):118-27.
  • Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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