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Printable Datasheet

Biotin (Vitamin B7 or Vitamin H) Antibody [BTN399]

In Stock
Catalog Number Formulation Size Price
MSM2-399-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-399-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-399-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin Mouse Monoclonal Antibody (BTN399).

Formalin-fixed, paraffin-embedded human kidney. Endogenous biotin stained with Biotin Mouse Monoclonal Antibody (BTN399).

SDS-PAGE Analysis of Purified Biotin Mouse Monoclonal Antibody (BTN399). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Biotin Mouse Monoclonal Antibody (BTN399). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
All species
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Biotinylated proteins in solution or on tissues.
Immunogen
Biotinylated sheep immunoglobulin
Alternate Names
N/A, N/A, Vitamin B7 or Vitamin H

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
BTN399
Chromosome Location
Not Applicable
Mol. Weight of Antigen
244Da

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Uccellini MB,et al.2008.J. Immunol.181:5875.
  • Green NM,et al.2012.J Biol Chem.287:39789

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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