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Human Nucleolar Antigen (Marker For Human Cells) Antibody [NM95]

In Stock
Catalog Number Formulation Size Price
MSM2-95-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-95-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-95-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Nucleolar Antibody in Immunohistochemistry (IHC (P))

Nucleolar Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleolar Mouse Monoclonal Antibody (NM95).

Nucleolar Antibody in Immunohistochemistry (IHC (P))

Nucleolar Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Mouse Monoclonal Antibody (NM95).

Nucleolar Antibody in Immunofluorescence (IF)

Nucleolar Antibody in Immunofluorescence (IF)

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488 Conjugate of Nucleolar Mouse Monoclonal Antibody (NM95).

Nucleolar Antibody in Flow Cytometry (FC)

Nucleolar Antibody in Flow Cytometry (FC)

Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleolar MAb (NM95).

Nucleolar Antibody in SDS-PAGE

Nucleolar Antibody in SDS-PAGE

SDS-PAGE Analysis Purified Nucleolar Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody

Nucleolar Antibody in Flow Cytometry (FC)

Nucleolar Antibody in Flow Cytometry (FC)

Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

Nucleolar Antibody in Immunofluorescence (IF)

Nucleolar Antibody in Immunofluorescence (IF)

Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 /
Cellular Localization
Nucleolus
Gene Name
N/A
Positive Control
Any human cells. Tissues.
Immunogen
Nuclei of myeloid leukemia biopsy cells
Alternate Names
N/A, N/A, Not Known

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
NM95
Chromosome Location
Not Known
Mol. Weight of Antigen
Not Known

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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