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Printable Datasheet

Cytokeratin 5/6/18 Antibody [SPM267]

In Stock
Catalog Number Formulation Size Price
MSM36X-2036-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM36X-2036-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM36X-2036-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267).

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267).

SDS-PAGE Analysis of Purified Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Cytokeratin, 5/6/18 Mouse Monoclonal Antibody (SPM267). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as cytokeratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. This MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labeled whether they are ectodermal, mesodermal, or endodermal in origin. This MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Cow, Human, Rat
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
HeLa, HepG2 or PC12 cells. Tonsil or Skin.
Immunogen
Detergent-insoluble fraction of psoriatic human epidermis
Alternate Names
N/A, N/A, CK5; Keratin-5; KRT5; Cytoskeletal 6,; KRT6, CK18; CYK18; K18; Keratin-18; KRT18

Database Links

Entrez Gene ID
Multiple
SwissProt
N/A

Additional Information

Clone
SPM267
Chromosome Location
Multiple
Mol. Weight of Antigen
45.58kDa

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Lane EB, et. al. Annals of the New York Academy of Sciences, 1985, 455:241-58.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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