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Nuclear Antigen (Pan-Nuclear Marker) Antibody [NM106]

In Stock
Best Seller
Catalog Number Formulation Size Price
MSM4-106-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM4-106-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM4-106-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Nuclear Antigen (Pan-Nuclear Marker) Antibody

Formalin-fixed, paraffin-embeddedhuman tonsil stained with Pan-Nuclear AntigenMonoclonal Antibody (NM106).

Nuclear Antigen (Pan-Nuclear Marker) Antibody - Image 2

Formalin-fixed, paraffin-embeddedhuman tonsil stained with Pan-Nuclear AntigenMonoclonal Antibody (NM106).

Nuclear Antigen (Pan-Nuclear Marker) Antibody - Image 3

Formalin-fixed, paraffin-embeddedratcolon stained with Pan-Nuclear AntigenMonoclonal Antibody (NM106).

Nuclear Antigen (Pan-Nuclear Marker) Antibody - Image 4

Formalin-fixed, paraffin-embeddedratlung stained with Pan-Nuclear AntigenMonoclonal Antibody (NM106).

Nuclear Antigen (Pan-Nuclear Marker) Antibody - Image 5

Immunofluorescentstaining of PFA-fixedHeLa cells with Pan-Nuclear Antigen Monoclonal Antibody (NM106)followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).

Nuclear Antigen (Pan-Nuclear Marker) Antibody - Image 6

SDS-PAGE Analysis Purified Pan-Nuclear Antigen Monoclonal Antibody (NM106). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse, Rat
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
All human cells. Tonsil
Immunogen
Nuclei of HL60 cells
Alternate Names
N/A, N/A, Nuclear Antigen

Database Links

Entrez Gene ID
Not Known
SwissProt
N/A

Additional Information

Clone
NM106
Chromosome Location
Not Known
Mol. Weight of Antigen
Not Known

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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