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GCDFP-15 (Gross Cystic Disease Fluid Protein 15) (Breast Marker) Antibody [PIP/7477]

In Stock
Catalog Number Formulation Size Price
5304-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5304-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5304-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis Purified GCDFP-15 Mouse Monoclonal Antibody (PIP/7477). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis Purified GCDFP-15 Mouse Monoclonal Antibody (PIP/7477). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with GCDFP-15 Mouse Monoclonal Antibody (PIP/7477). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with GCDFP-15 Mouse Monoclonal Antibody (PIP/7477). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen-controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis. �

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
AKT Signaling
Isotype / Light Chain
IgG /
Cellular Localization
Cytoplasm (Golgi bodies). Secreted.
Gene Name
PIP
Positive Control
HepG2 cells. Breast or Pancreas.
Immunogen
Recombinant fragment (around aa 41-146) of human GCDFP-15 protein (exact sequence is proprietary)
Alternate Names
Prolactin-inducible protein, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein, gp17, BRST-2; GCDFP-15; gp17; GPIP4; Gross cystic disease fluid protein 15; Prolactin-induced protein (PIP); Prolactin-inducible protein (PIP); Secretory actin-binding protein (SABP)

Database Links

Entrez Gene ID
5304
SwissProt
P12273

Additional Information

Clone
PIP/7477
Chromosome Location
7q34
Mol. Weight of Antigen
15kDa

Related Antibodies

View All PIP Antibodies

Key References

  • Wick MR.,et al. Hum Pathol.1989 Mar;20(3):281-7.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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