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Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody [LPFS2/8574R]

In Stock
Catalog Number Formulation Size Price
939-RBM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
939-RBM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
939-RBM12-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human lymph node stained with CD27 Rabbit Recombinant Monoclonal Antibody (LPFS2/8574R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human lymph node stained with CD27 Rabbit Recombinant Monoclonal Antibody (LPFS2/8574R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

CD27 Antibody in Western Blot (WB)

CD27 Antibody in Western Blot (WB)

Western Blot Analysis of Raji and Ramos lysate using CD27 Rabbit Recombinant Monoclonal Antibody (LPFS2/8574R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, AKT Signaling, B Cell Markers, Cytokine Signaling
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface.
Gene Name
CD27
Positive Control
Human peripheral blood lymphocytes. Human tonsil and lymph node.
Immunogen
Recombinant human full-length CD22 protein (exact sequence is proprietary)
Alternate Names
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7

Database Links

Entrez Gene ID
939
SwissProt
P26842

Additional Information

Clone
LPFS2/8574R
Chromosome Location
12p13
Mol. Weight of Antigen
120kDa

Related Antibodies

View All CD27 Antibodies

Functions

  • Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Key References

  • De Rie MA,Cairo I,Van Lier RA,Bos JD. Expression of the T-cell activation antigens CD27 and CD28 in normal and psoriatic skin. Clin Exp Dermatol.1996 Mar;21(2):104-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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