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Recombinant Occludin (OCLN) (Tight Junctions Marker) Antibody [rOCLN/8776]

In Stock
Catalog Number Formulation Size Price
100506658-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
100506658-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
100506658-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SDS-PAGE Analysis of Purified Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776) Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776) Confirmation of Purity and Integrity of Antibody.

Occludin Antibody in Western Blot (WB)

Occludin Antibody in Western Blot (WB)

Western Blot Analysis of human kidney tissue lysate using Occludin Recombinant Mouse Monoclonal Antibody (rOCLN/8776).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
BBB VCAM-1 Signaling, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
OCLN
Positive Control
HepG2 cells. Human kidney.
Immunogen
Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary)
Alternate Names
Occludin, BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin

Database Links

Entrez Gene ID
100506658
SwissProt
Q16625

Additional Information

Clone
rOCLN/8776
Chromosome Location
5q13.2
Mol. Weight of Antigen
60-82kDa

Related Antibodies

View All OCLN Antibodies

Functions

  • May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

Key References

  • Furuse, M., et al. 1993. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123: 1777-1788.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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