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Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody [rPCNA/8858]

In Stock
Catalog Number Formulation Size Price
5111-MSM11-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5111-MSM11-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5111-MSM11-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

PCNA Antibody in SDS-PAGE

PCNA Antibody in SDS-PAGE

SDS-PAGE Analysis Purified PCNA Recommbinant Mouse Monoclonal Antibody (rPCNA/8859). Confirmation of Purity and Integrity of Antibody.

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody in Western Blot (WB)

Western Blot Analysis of A-431, 293T and Hep G2 cell lysates using PCNA Recombinant Mouse Monoclonal Antibody (rPCNA/8858).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Nuclear Marker, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus. Cytoplasm.
Gene Name
PCNA
Positive Control
A-431, 293T, Hep G2, U937 cells.|Tonsil or reactive lymph node. SKBr3
Immunogen
Recombinant full-length human PCNA protein
Alternate Names
Proliferating cell nuclear antigen, Cyclin, Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein

Database Links

Entrez Gene ID
5111
SwissProt
P12004

Additional Information

Clone
rPCNA/8858
Chromosome Location
20p12.3
Mol. Weight of Antigen
36kDa

Related Antibodies

View All PCNA Antibodies

Functions

  • Auxiliary protein of DNA polymerase delta and epsilon, is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand (PubMed:35585232). Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).

Key References

  • Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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