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NTRK2 (Neurotrophic Receptor Tyrosine Kinase 2) Antibody [NTRK2/7928]

In Stock
Catalog Number Formulation Size Price
4915-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4915-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4915-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
NTRK2 (Neurotrophic Receptor Tyrosine Kinase 2) Antibody

Formalin-fixed, paraffin-embedded human brain stained with NTRK2 / TRKb Mouse Monoclonal Antibody (NTRK2/7928). Inset: PBS instead of primary antibody; secondary only negative control.

NTRK2 (Neurotrophic Receptor Tyrosine Kinase 2) Antibody - Image 2

Formalin-fixed, paraffin-embedded human thyroid stained with NTRK2 / TRKb Mouse Monoclonal Antibody (NTRK2/7928). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Neuroscience, AKT Signaling, MAPK Signaling, Signal Transduction
Isotype / Light Chain
IgG2 / Lambda
Cellular Localization
Cell surface.
Gene Name
NTRK2
Positive Control
Human brain tissue.
Immunogen
Recombinant fragment (around aa250-450) of human NTRK2 protein (exact sequence is proprietary)
Alternate Names
BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, AI848316; BDNF tropomyosine receptor kinase B; BDNF/NT-3 growth factors receptor; Brain derived neurotrophic factor receptor; C030027L06Rik; GP145 TrkB; GP95 TrkB; Neurotrophin receptor tyrosine kinase type 2; Obesity, hyperphagia, and developmental delay, included; RATTRKB1; Tropomyosin related kinase B; Tyrosine receptor kinase B

Database Links

Entrez Gene ID
4915
SwissProt
Q16620--------

Additional Information

Clone
NTRK2/7928
Chromosome Location
9q21.33
Mol. Weight of Antigen
95-145kDa

Related Antibodies

View All NTRK2 Antibodies

Functions

  • Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed:7574684, PubMed:15494731). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed:15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.

Key References

  • Camer, D., et al. 2015. Prog. Neuropsychopharmacol. Biol. Psychiatry 59: 68-75.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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