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Recombinant Desmoglein-3 (Squamous Cell Marker) Antibody [DSG3/8613R]

In Stock
Catalog Number Formulation Size Price
1830-RBM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1830-RBM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1830-RBM15-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified DSG3 Recombinant Rabbit Monoclonal Antibody (DSG3/8613R). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified DSG3 Recombinant Rabbit Monoclonal Antibody (DSG3/8613R). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human skin stained with Desmoglein-3 Recombinant Rabbit Monoclonal Antibody (DSG3/8613R). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human skin stained with Desmoglein-3 Recombinant Rabbit Monoclonal Antibody (DSG3/8613R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell surface
Gene Name
DSG3
Positive Control
A431 cells. Lung squamous cell carcinoma.
Immunogen
Recombinant fragment (around aa300-500) of human DSG3 protein (exact sequence is proprietary)
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
1830
SwissProt
P32926Human

Additional Information

Clone
DSG3/8613R
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Related Antibodies

View All DSG3 Antibodies

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Key References

  • Mahoney, MG; Hu, Y; Brennan, D; Bazzi, H; Christiano, AM; Wahl, JK III. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol. 2006, 15(2):101-9.
  • Wahl JK III. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21(1):37-44.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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