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beta Amyloid Antibody [APP/4469]

In Stock
Catalog Number Formulation Size Price
351-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
351-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
351-MSM9-P1ABX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
β-Amyloid Antibody in SDS-PAGE

β-Amyloid Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Beta Amyloid Mouse Monoclonal Antibody (APP/4469). Confirmation of Purity and Integrity of Antibody.

β-Amyloid Antibody in Immunohistochemistry (IHC (P))

β-Amyloid Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human brain stained with Beta Amyloid Mouse Monoclonal Antibody (APP/4469). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the β-Amyloid and Amyloid A4 proteins, which are present in human platelets. Amyloid deposition is associated with type II diabetes, Down syndrome and a variety of neurological disorders, including Alzheimer s disease. The Amyloid precursor protein (APP) undergoes alternative splicing, resulting in several isoforms. Proteolytic cleavage of APP leads to the formation of the 4 kDa Amyloid β/A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane-bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer s disease patients.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Immunology, Neuroscience, Cytokine Signaling, Defective Intrinsic Apoptosis, Infectious Disease, Ovarian Cancer, Signal Transduction
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane. Membrane > clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits.
Gene Name
APP
Positive Control
Alzheimer's diseased brain. Amyloidosis.
Immunogen
Recombinant fragment (around aa1-100) of human APP protein (exact sequence is proprietary). Recognizes an epitope in the region 8-16 of the amyloid precursor protein.
Alternate Names
Amyloid-beta precursor protein | N-APP | Soluble APP-alpha | Soluble APP-beta | C99 | Amyloid-beta protein 42 | Amyloid-beta protein 40 | C83 | P3(42) | P3(40) | C80 | Gamma-secretase C-terminal fragment 59 | Gamma-secretase C-terminal fragment 57 | Gamma-secretase C-terminal fragment 50 | C31, ABPP, APPI, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, Beta-secretase C-terminal fragment, Beta-APP42, Beta-APP40, Alpha-secretase C-terminal fragment, Amyloid intracellular domain 59, Gamma-CTF(59), Amyloid intracellular domain 57, Gamma-CTF(57), Amyloid intracellular domain 50, Gamma-CTF(50), CVAP; AAA; AICD-50; PN2; 50; Beta-APP42; AID; S-APP-alpha; 57; AD1; PN-II; Beta-APP40; 42; 40; APPI; Alzheimer disease amyloid protein; PreA4; ABETA; Amyloid intracellular domain 50; 57; 59 AICD-59; S-APP-beta; APP; AICD-57; ABPP; Protease nexin-II; Cerebral vascular amyloid peptide

Database Links

Entrez Gene ID
351
SwissProt
P05067

Additional Information

Clone
APP/4469
Chromosome Location
21q21.3
Mol. Weight of Antigen
4-46kDa (β-Amyloid); 100-125kDa (Amyloid A4)

Related Antibodies

View All APP Antibodies

Functions

  • Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis (PubMed:25122912). Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons (PubMed:17062754, PubMed:23011729). Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.
  • Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts.
  • Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain.
  • The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
  • N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

Key References

  • Dukers DF, et al. J Clin Pathol 1999; 52:12936.
  • Kanavaros P, et al. Anticancer Res 1999; 19:120916.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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