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Recombinant Androgen Receptor V7 (Marker of Androgen Dependence) Antibody [DHTR.V7/9125R]

In Stock
Catalog Number Formulation Size Price
367-RBM19-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
367-RBM19-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
367-RBM19-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AR Recombinant Rabbit Monoclonal Antibody (DHTR.V7/9125R). HIER: Tris/EDTA, pH9.0, 95°C/45min. 2°CAb: HRP-Poly:30min. DAB:5min.

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AR Recombinant Rabbit Monoclonal Antibody (DHTR.V7/9125R). HIER: Tris/EDTA, pH9.0, 95°C/45min. 2°CAb: HRP-Poly:30min. DAB:5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

AR V7 belongs to the nuclear hormone receptor family. It contains the nuclear receptor DNA binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate the expression of eukaryotic genes affecting cell proliferation and differentiation in target tissues. The activity of transcription factors is regulated by binding co-activators and co-repressors. This target is a splice variant of the androgen receptor that lacks the C-terminal androgen-binding site. AR-V7 plays an important role in the occurrence, development and drug resistance of prostate cancer.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
AKT Signaling, Signal Transduction, Transcription Factors
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Nucleus.
Gene Name
AR
Positive Control
Breast|LNCap cells. Human testis|prostate or prostate carcinoma.
Immunogen
Recombinant fragment (around aa600-920) of human AR protein (AR-V7 specific) (exact sequence is proprietary)
Alternate Names
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AIS; Dihydrotestosterone receptor (DHTR); HUMARA; HYSP1; Kennedy disease (KD); Nuclear receptor subfamily 3 group C member 4 (NR3C4); SMAX1; Spinal and bulbar muscular atrophy (SBMA); Testicular Feminization (TFM)

Database Links

Entrez Gene ID
367
SwissProt
P10275

Additional Information

Clone
DHTR.V7/9125R
Chromosome Location
Xq12
Mol. Weight of Antigen
110kDa

Related Antibodies

View All AR Antibodies

Functions

  • Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
  • Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Key References

  • Chen W et al. J Cell Biochem 120:14055-14064 (2019).
  • Honda M et al. Prostate 79:1043-1052 (2019).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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