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CD203c / ENPP3 (Basophil Marker) Antibody [ENPP3/9892]

In Stock
Catalog Number Formulation Size Price
5169-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5169-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5169-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD203c Antibody in Immunohistochemistry (IHC (P))

CD203c Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human kidney stained with CD203c / ENPP3 Mouse Monoclonal Antibody (ENPP3/9892). Inset: PBS instead of primary antibody; secondary only negative control.

CD203c Antibody in SDS-PAGE

CD203c Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD203c Mouse Monoclonal Antibody (ENPP3/9892). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Ecto-nucleotide pyrophosphatase/phosphodiesterase-I enzymes (E-NPP), a group of type II transmembrane proteins, cleave phosphodiester and phosphosulfate bonds in deoxynucleotides, NAD, and nucleotide sugars. There exist three closely related proteins in mammalian species: E-NPP1 (PC-1), E-NPP2 (PDNP2), and E-NPP-3, also known as CD203c, each expressed in different cells or at different locations in the same cells. Basophils, a type of major proinflammatory effector cells involved in diverse pathologic reactions, exhibit CD203c expression. For instance, CD203c expression increases in response to a variety of allergens, including cat dander, latex, and bee and wasp venom. Hovever, these allergies are not the only condition associated with CD203c upregulation; CD203c upregulation may also serve as a tumor marker for colon carcinoma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Epigenetics, Metabolism, Signal Transduction
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Secreted
Gene Name
ENPP3
Immunogen
Recombinant fragment (around aa300-600) of human ENPP3 protein (exact sequence is proprietary)
Alternate Names
ectonucleotide pyrophosphatase/phosphodiesterase family member 3, Alkaline phosphodiesterase I antibody; ARHR2 antibody; B10 antibody; CD203c antibody; CD203c antigen antibody; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3) antibody; E NPP 3 antibody; E-NPP 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase 3 antibody; Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 antibody; ENPP3 antibody ENPP3_HUMAN antibody; gp130RB13 6 antibody; gp130RB136 antibody; M6S1 antibody; NPP1 antibody; NPP3 antibody; NPPase antibody; NPPS antibody; Nucleotide pyrophosphatase antibody; PC 1 antibody; PC-1 antibody; PCA1 antibody; PD Ibeta antibody; PD-Ibeta antibody; PDNP1 antibody; PDNP3 antibody; Phosphodiesterase I beta antibody; Phosphodiesterase I/nucleotide pyrophosphatase 3 antibody; phosphodiesterase i/nucleotide pyrophosphatase beta antibody, B10; NPP3; PDNP3; CD203c; PD-IBETA

Database Links

Entrez Gene ID
5169
SwissProt
O14638

Additional Information

Clone
ENPP3/9892
Chromosome Location
6q23.2
Mol. Weight of Antigen
100kDa

Related Antibodies

View All ENPP3 Antibodies

Functions

  • Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.

Key References

  • Binder, M., et al. 2002. Individual hymenoptera venom compounds induce upregulation of the basophil activation marker ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c) insensitized patients. Int. Arch. Allergy Immunol. 129: 160-168.
  • Yano, Y., et al. 2003. Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-3 (E-NPP3/CD203c/PD-Ib/B10/gp130RB13-6) in human colon carcinoma. Int. J. Mol. Med. 12: 763-766.
  • Majlesi, Y., et al. 2003. Cerivastatin and atorvastatin inhibit IL-3-dependent differentiation and IgE-mediated histamine release in human basophils and downmodulate expression of the basophil-activation antigen CD203c/E-NPP3. J. Leukoc. Biol. 73: 107-117.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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