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Glucose 6-Phosphate Isomerase Antibody [GPI/2439]

In Stock
Catalog Number Formulation Size Price
2821-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2821-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2821-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GPI Antibody in Western Blot (WB)

GPI Antibody in Western Blot (WB)

Western blot analysis of Human kidney tissue lysate using Glucose 6-Phosphate Isomerase Mouse Monoclonal Antibody (GPI/2439).

GPI Antibody in Immunohistochemistry (IHC (P))

GPI Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with Glucose 6-Phosphate Isomerase Mouse Monoclonal Antibody (GPI/2439). Inset: PBS instead of primary antibody; secondary only negative control.

GPI in SDS-PAGE

GPI in SDS-PAGE

SDS-PAGE Analysis of Purified GPI Mouse Monoclonal Antibody (GPI/2439). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Besides it’s role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Colon Cancer, Transcription Factors
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Secreted
Gene Name
GPI
Positive Control
PC3, T47D, HepG2, MCF-7 or A549 cells. Human kidney, brain, pancreas or thyroid carcinoma.
Immunogen
Recombinant human full-length GPI protein
Alternate Names
Glucose-6-phosphate isomerase, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF; aurocrine motility factor; EC 5.2.1.9; GNPI; Gpi1; hexose monophosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphosaccharomutase; SA-36; sperm antigen 36

Database Links

Entrez Gene ID
2821
SwissProt
P06744

Additional Information

Clone
GPI/2439
Chromosome Location
19q13.11
Mol. Weight of Antigen
55kDa

Related Antibodies

View All GPI Antibodies

Functions

  • In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed:11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:3352745, PubMed:11004567). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:3352745, PubMed:11004567).

Key References

  • Beutler, E., et al. 1997.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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