ER-beta-1 (Estrogen Receptor beta-1) Antibody [ERb455]

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ER-beta1 Antibody in Flow Cytometry (FC)

Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ERb455).

Estrogen Receptor beta 1 Antibody in SDS-PAGE

SDS-PAGE Analysis Purified Estrogen Receptor beta1 Mouse Monoclonal Antibody (ERb455). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Western Blot (WB)

2-4ug/ml

Western Blot Protocol

Summary

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human

Research Areas

Breast Cancer, Cardiovascular, Infectious Disease, Nuclear Marker, Signal Transduction, Transcription Factors

Isotype / Light Chain

IgG2a / Kappa

Cellular Localization

Nucleus

Gene Name

ESR2

Positive Control

MCF-7 cells. Human breast or ovarian tissue.

Immunogen

C-terminus fragment of recombinant human estrogen receptor beta protein

Alternate Names

Estrogen receptor beta, Nuclear receptor subfamily 3 group A member 2, Erb, ESR BETA, ESR2, ESRB, ESTRB, estrogen nuclear receptor beta variant a, estrogen nuclear receptor beta variant b, estrogen receptor 2 (ER beta), estrogen receptor beta 4

Database Links

Entrez Gene ID

2100

SwissProt

Q92731

Additional Information

Clone

ERb455

Chromosome Location

14q23.2

Mol. Weight of Antigen

53-59kDa

Functions

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).
Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

Key References

Skliris GP et. al. J Pathol 2002;197:155-62.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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