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CEACAM1 (CEA Cell Adhesion Molecule 1) / CD66a Antibody [CEACAM1/12665]

In Stock
Catalog Number Formulation Size Price
634-MSM45-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
634-MSM45-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
634-MSM45-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CEACAM1 / CD66a Antibody in Immunohistochemistry (IHC (P))

CEACAM1 / CD66a Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon stained with CEACAM1 Mouse Monoclonal Antibody (CEACAM1/12665). Inset: PBS instead of primary antibody; secondary only negative control.

CEACAM1 Antibody in Western Blot (WB)

CEACAM1 Antibody in Western Blot (WB)

Western Blot Analysis of SK-MEL-28 lysate using CEACAM1 Mouse Monoclonal Antibody (CEACAM1/12665).

CEACAM1 Antibody in SDS-PAGE

CEACAM1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CEACAM1 Mouse Monoclonal Antibody (CEACAM1/12665). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Carcinoembryonic antigen (CEA) is one of the most commonly used tumor markers in serum immunoassay determinations of carcinoma. Members of the CEACAM (carcinoembryonic antigen-related cell adhesion molecule) family contain a single N domain, with structural homology to the immunoglobulin variable domains, followed by a variable number of immunoglobulin constantlike A and/or B domains. CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1), also known as BGP or BGP1, is a 526 amino acid protein that exists as seven alternatively spliced isoforms, some of which localize to the cell membranes, while others are secreted. One of several members of the CEACAM family, CEACAM1 contains one Ig-like V-type domain and three Ig-like C2-type domains and is thought to play a role in a variety of cellular activities, including angiogenesis, apoptosis, arrangement of tissue threedimensional structure and modulation of innate and adaptive immune responses. Additionally, CEACAM1 is underexpressed in colorectal cancers, suggesting a role in tumor suppression.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immunology, Hematopoietic Stem Cells
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CEACAM1
Positive Control
Human colon, liver, endometrium or hepatic carcinoma tissues. SK-MEL-28 cells.
Immunogen
Recombinant fragment (around aa 1-200) of human CEACAM1 protein (exact sequence is proprietary)
Alternate Names
Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1, Antigen CD66; BGP-1; BGPI; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD66a; CEACAM1; meconium antigen 100

Database Links

Entrez Gene ID
634
SwissProt
P13688

Additional Information

Clone
CEACAM1/12665
Chromosome Location
19q13.2
Mol. Weight of Antigen
90-180kDa

Related Antibodies

View All CEACAM1 Antibodies

Functions

  • Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Plays a role as coinhibitory receptor in immune response, insulin action and functions also as an activator during angiogenesis (PubMed:18424730, PubMed:23696226, PubMed:25363763). Its coinhibitory receptor function is phosphorylation- and PTPN6 -dependent, which in turn, suppress signal transduction of associated receptors by dephosphorylation of their downstream effectors. Plays a role in immune response, of T cells, natural killer (NK) and neutrophils (PubMed:18424730, PubMed:23696226). Upon TCR/CD3 complex stimulation, inhibits TCR-mediated cytotoxicity by blocking granule exocytosis by mediating homophilic binding to adjacent cells, allowing interaction with and phosphorylation by LCK and interaction with the TCR/CD3 complex which recruits PTPN6 resulting in dephosphorylation of CD247 and ZAP70 (PubMed:18424730). Also inhibits T cell proliferation and cytokine production through inhibition of JNK cascade and plays a crucial role in regulating autoimmunity and anti-tumor immunity by inhibiting T cell through its interaction with HAVCR2 (PubMed:25363763). Upon natural killer (NK) cells activation, inhibit KLRK1-mediated cytolysis of CEACAM1-bearing tumor cells by trans-homophilic interactions with CEACAM1 on the target cell and lead to cis-interaction between CEACAM1 and KLRK1, allowing PTPN6 recruitment and then VAV1 dephosphorylation (PubMed:23696226). Upon neutrophils activation negatively regulates IL1B production by recruiting PTPN6 to a SYK-TLR4-CEACAM1 complex, that dephosphorylates SYK, reducing the production of reactive oxygen species (ROS) and lysosome disruption, which in turn, reduces the activity of the inflammasome. Down-regulates neutrophil production by acting as a coinhibitory receptor for CSF3R by down-regulating the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R (By similarity). Also regulates insulin action by promoting INS clearance and regulating lipogenesis in liver through regulating insulin signaling (By similarity). Upon INS stimulation, undergoes phosphorylation by INSR leading to INS clearance by increasing receptor-mediated insulin endocytosis. This inernalization promotes interaction with FASN leading to receptor-mediated insulin degradation and to reduction of FASN activity leading to negative regulation of fatty acid synthesis. INSR-mediated phosphorylation also provokes a down-regulation of cell proliferation through SHC1 interaction resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 and phosphatidylinositol 3-kinase pathways (By similarity). Functions as activator in angiogenesis by promoting blood vessel remodeling through endothelial cell differentiation and migration and in arteriogenesis by increasing the number of collateral arteries and collateral vessel calibers after ischemia. Also regulates vascular permeability through the VEGFR2 signaling pathway resulting in control of nitric oxide production (By similarity). Down-regulates cell growth in response to EGF through its interaction with SHC1 that mediates interaction with EGFR resulting in decrease coupling of SHC1 to the MAPK3/ERK1-MAPK1/ERK2 pathway (By similarity). Negatively regulates platelet aggregation by decreasing platelet adhesion on type I collagen through the GPVI-FcRgamma complex (By similarity). Inhibits cell migration and cell scattering through interaction with FLNA; interfers with the interaction of FLNA with RALA (PubMed:16291724). Mediates bile acid transport activity in a phosphorylation dependent manner (By similarity). Negatively regulates osteoclastogenesis (By similarity).
  • Cell adhesion protein that mediates homophilic cell adhesion in a calcium-independent manner (By similarity). Promotes populations of T cells regulating IgA production and secretion associated with control of the commensal microbiota and resistance to enteropathogens (By similarity).

Key References

  • Miura M, et al. Exp Cell Res 1996;226:126-132.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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