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Recombinant ALCAM (Activated Leukocyte Cell Adhesion Molecule) / CD166 Antibody [rALCAM/9811]

In Stock
Catalog Number Formulation Size Price
214-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
214-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
214-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ALCAM / CD166 Antibody in Immunohistochemistry (IHC (P))

ALCAM / CD166 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human parathyroid hormone stained with ALCAM / CD166 Mouse Monoclonal Antibody (rALCAM/9811). Inset: PBS instead of primary antibody; secondary only negative control.

ALCAM Antibody in Western Blot (WB)

ALCAM Antibody in Western Blot (WB)

Western blot analysis of A-549 and A-431 cell lysates using CD166 Mouse Monoclonal Antibody (rALCAM/9811).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Activated leukocyte cell adhesion molecule (ALCAM), expressed on activated leukocytes, T cells, B cells and monocytes, is a member of the immunoglobulin superfamily (IgSF) and identified as a CD6 ligand. CD6 is a type I membrane protein in the scavenger receptor cysteine rich protein superfamily that acts in T cell adhesion and costimulation. ALCAM mediates thymocyte-thymic epithelial cell adhesion via homophilic (ALCAM-ALCAM) and heterophilic (ALCAM-CD6) interactions involving a previously undescribed protein-protein interaction between a member of the scavenger receptor cysteine rich protein superfamily and the immunoglobulin superfamily.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immuno Oncology, Immunology
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane
Gene Name
ALCAM
Positive Control
Human tonsil, spleen, colon or parathyroid hormone.
Immunogen
Recombinant full-length human CD166 protein
Alternate Names
CD166 antigen, Activated leukocyte cell adhesion molecule, CD166 antigen; Activated leukocyte cell adhesion molecule; ALCAM; MEMD

Database Links

Entrez Gene ID
214
SwissProt
Q13740

Additional Information

Clone
rALCAM/9811
Chromosome Location
3q13.11
Mol. Weight of Antigen
100-105kDa

Related Antibodies

View All ALCAM Antibodies

Functions

  • Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed:15048703, PubMed:15496415, PubMed:16352806, PubMed:23169771, PubMed:24945728, PubMed:7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed:15048703, PubMed:16352806, PubMed:24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed:15294938, PubMed:16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed:15496415, PubMed:16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed:29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed:23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:15496415, PubMed:23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation. Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

Key References

  • Yasen, M., Fei, Q., et al. (2013). Changes of number of cells expressing proliferation and progenitor cell markers with age in rabbit intervertebral discs. Acta biochimica et biophysica Sinica, 45(5), 368–376. https://doi.org/10.1093/abbs/gmt019
  • Thakkar, A., Wang, B., Picon-Ruiz, et al. (2016). Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer. Breast cancer research and treatment, 157(1), 77–90. https://doi.org/10.1007/s10549-016-3807-y
  • Fang, Z., Zeng, J. J., et al. (2023). Expression of ALCAM in Clinical Colon Cancer and Relationship With Patients' Treatment Responses. In vivo (Athens, Greece), 37(3), 1117–1128. https://doi.org/10.21873/invivo.13187
  • Yang, Y. M., Ruge, F., et al. (2023). ALCAM, Activated Leukocyte Cell Adhesion Molecule, in Clinical Gastric Cancer and Patient's Response to Chemotherapies. Anticancer research, 43(4), 1463–1475. https://doi.org/10.21873/anticanres.16295

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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